By S. Morse
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Sonication was accomplished using an Ultrasonik 300 (Neytech, Burlington, NJ), while vortexing was accomplished using a VortexGenie 1 (Scientific Industries, Bohemia, NY). 1 digital camera. An initial stock solution of 20 mg of DPA in 20 mL HPLC grade water (Fischer Scientific, Fair Lawn, NJ) was prepared for the pH study. 00 buffer solutions (Fischer Scientific). For all pH measurements a single 2-mL glass vial coated with silver-doped sol-gel was used (Simple SERS Sample Vial, Real-Time Analyzers, Inc.
Pre-weighed spore particles were placed on a glass plate for these measurements. Final spore measurements employed room temperature acetic acid to digest the spores. Pre-weighed spores were used to prepare a stock solution for calibration using a counting grid (see below), from which a known number of spores were dried on a glass plate. For both digesting chemicals, after 1 min exposure, the degraded spore sample was drawn into a SER-active capillary (Simple SERS Sample Capillary, RTA) for measurement.
Furthermore, it is usually found that RS spectra of analytes in solution more closely match the SER spectra than in the solid-state. However, it is usually beneficial to acquire and examine both when making assignments. Since DPA dissolves in water only sparingly, 1N KOH was used to dissolve 80 mg/mL. The RS spectrum of the solution phase is largely the same as the solid phase except for some minor changes in peak frequencies, intensities, and widths (Table 1). Notably, the 760 cm-1 peak in the solid phase is completely absent in the solution phase, while a new peak at 1386 cm-1 appears in the solution phase.